2 edition of Functional characterization of PRDM12, a gene recurrently deleted during t(9;22) rearrangements in chronic myeloid leukemia patients. found in the catalog.
Functional characterization of PRDM12, a gene recurrently deleted during t(9;22) rearrangements in chronic myeloid leukemia patients.
Stefanie A. Turley
Written in English
Constitutive activation of BCR-ABL tyrosine kinase is the hallmark of CML in 95% of patients. The reciprocal fusion product, ABL-BCR, is deleted in 20% of patients. Studies have revealed a 120 kb deletion centromeric of ABL encompassing: PRDM12, a putative histone methyltransferase, and EXOSC2, a 3"-5" exoribonuclease.PRDM12 is one of 16 PR family members. PRDM12 consists of a PR domain and 3 zinc fingers. PR domains are thought to function as histone methyltransferases (HMT) as they share sequence similarity to the SET domain, known histone methyltransferases. The zinc fingers are important in DNA binding and protein-protein interactions. The PR domain of PRDM12 does not possess intrinsic HMT activity. Interestingly, PRDM12 is found associated with chromatin, suggesting it may be important in recruiting a complex of proteins involved in repression of transcription, as does another PR family member, PRDM1/PRDI-BF1/BLIMP-1. The involvement of PRDM12 with chromatin suggests a possible role in transcription regulation. This may reveal its importance in a more aggressive form of CML.
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Abstract. Prdm genes encode transcription factors with a subtype of SET domain known as the PRDF1-RIZ (PR) homology domain and a variable number of zinc finger motifs. These genes are involved in a wide variety of functions during animal development. As most Prdm genes have been studied in vertebrates, especially in mice, little is known about the evolution of this gene family. PRDM (PRDI-BF1 and RIZ homology domain containing) protein family members are characterized by the presence of a PR domain and a variable number of Zn-finger repeats. Experimental evidence has shown that the PRDM proteins play an important role in gene expression regulation, modifying the chromatin structure either directly, through the intrinsic methyltransferase activity, or indirectly.
Publications for gene: PRDM12 were set to ; 19 Sep , Gel status: 4 panel promoted to version 1 Louise Daugherty (Genomics England Curator) 19th September Panel reviews were assessed, and panel was revised according to reviews and further curation. Gene: PRDM12 Green List (high evidence) PRDM12 (PR/SET domain 12) EnsemblGeneIds (GRCh38): ENSG EnsemblGeneIds (GRCh37 Five homozygous variants reported in five families from different geographical origins. In vitro functional evidence also provided. Created: 30 Aug , p.m. Mode of inheritance BIALLELIC, autosomal or.
Expression of wild-type human PRDM12 in Xenopus induced the expression of sensory neuronal markers, which was reduced using various human PRDM12 mutants. In Drosophila, we identified Hamlet as the functional PRDM12 homolog that controls nociceptive behavior in sensory neurons. This gene is located near the 1p breakpoint and has been shown to be specifically expressed in the t()(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks.
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This gene encodes a transcriptional regulator of sensory neuronal specification that plays a critical role in pain perception. The encoded protein contains an N-terminal PRDI-BF1 and RIZ homology (PR) domain, a SET domain, and three C-terminal C2H2 zinc finger DNA-binding domains.
Naturally occurring mutations in this gene are associated with congenital insensitivity to pain (CIP). a gene recurrently deleted during t book Chronic myeloid leukemia (CML; ) is a clonal hematopoietic stem cell disorder associated with the BCR ()/ABL1 fusion gene generated by the Philadelphia translocation t(9;22)(q34;q11).
Reid and Nacheva () noted that the PRDM12 gene lies within a region immediately 5-prime to ABL1 on chromosome 9q that is commonly deleted on the derivative chromosome 9 in.
PRDM12 gene PR/SET domain Open All Close All. The information on this page was automatically extracted from online scientific databases. Normal Function. From NCBI Gene: This gene encodes a transcriptional regulator of sensory neuronal specification that plays a critical role in pain perception.
The encoded protein contains an N-terminal. The PRDM12 gene encodes a transcriptional regulator that mediates the methylation of histone H3 lysine 9 (H3K9) through its interaction with a histone methyltransferase.
The proposed role of the PRDMmediated H3K9 methylation is transcriptional gene regulation during neural crest cells specification, specifically in the neurogenesis of. The gene view histogram is a graphical view of mutations across PRDM These mutations are displayed at the amino acid level across the full length of the gene by default.
Restrict the view to a region of the gene by dragging across the histogram to highlight the region of interest, or by using the sliders in the filters panel to the left.
The sensation of pain, temperature, and itch by neurons of the nociceptive lineage is essential for animal survival. Bartesaghi et al. report that the transcriptional regulator PRDM12 is indispensable in neural crest cells (NCCs) for the initiation of the sensory neuronal differentiation program that generates the entire nociceptive lineage.
The latter gene is a member of the closely related PR-domain-containing zinc-finger family, which appear to function as negative regulators of oncogenesis and include the tumour-associated genes.
Embryos were co-injected with Myc-Prdm12 mRNA and Control MO (5, 10, 20 ng/embryo) or Prdm12 MO (5, 10, 20 ng/embryo) at 2-cell stage and protein extracts were obtained from stage embryos. Cell Reports Report PRDM12 Is Required for Initiation of the Nociceptive Neuron Lineage during Neurogenesis Luca Bartesaghi,1,2,11 Yiqiao Wang,1,11 Paula Fontanet,1 Simone Wanderoy,1 Finja Berger,1,2 Haohao Wu,1 Natalia Akkuratova,3,4 Filipa Bouc¸anova,1,2 Jean-Jacques Me´dard,1,2 Charles Petitpre´,1 Mark A.
Landy,5 Ming-Dong Zhang,6 Philip Harrer, 7Claudia Stendel, Rolf. Prdm12 exerted antiproliferative effects in part through regulation of the G1 phase of the cell cycle. Furthermore, ectopic Prdm12 increased the expression of p27, with both the PR and ZF domains being necessary for its function.
1 Transcriptional regulator PRDM12 is essential for human pain perception Ya-Chun Chen1+, Michaela Auer-Grumbach2+, Shinya Matsukawa3, Manuela Zitzelsberger4, Andreas C. Themistocleous5, Tim M. Strom6,7, Chrysanthi Samara8, Adrian W. Moore9, Lily Ting-Yin Cho10, Gareth T. Young10, Caecilia Weiss4, Maria Schabhüttl2, Rolf Stucka4, Annina B.
Schmid5, Yesim Parman11, Luitgard Graul. Product # Description Species Reactivity Application Add to Cart: ABE95 Anti-PRDM12 Antibody Use Anti-PRDM12 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect PRDM12 also known as PR domain zinc finger prot arginine methyltransferase.
Nagy V, Cole T, Van Campenhout C, Khoung TM, Leung C, Vermeiren S, Novatchkova M, Wenzel D, Cikes D, Polyansky AA, Kozieradzki I, Meixner A, Bellefroid EJ, Neely GG, Penninger JMNagy V, et al. Cell Cycle, PMID ; A potential role for PRDM12 in the pathogenesis of chronic myeloid leukaemia with derivative chromosome 9 deletion.
Plasmid PRDM12 from Dr. Cheryl Arrowsmith's lab contains the insert PRDM12_ This plasmid is available through Addgene. 1. Introduction. PR domain containing protein 1 (Prdm1), also known as the β-interferon gene positive-regulatory domain I binding factor (PRDI-BF1) or B lymphocyte-induced maturation protein-1 (Blimp-1), is a transcriptional repressor that performs multiple important functions in cell fate and differentiation during immune responses and embryonic development (Keller and Maniatis.
Summary of PRDM12 expression in human tissue. Estimation of protein expression could not be performed. View primary data. Expression of PRDM12 in cancer tissue.
The cancer tissue page shows antibody staining of the protein in 20 different cancers. Entrez_Gene (NCBI) PRDM12 PR/SET domain Aliases: HSAN8; PFM9: GeneCards (Weizmann) PRDM Ensembl hg19 (Hinxton) ENSG [Gene_View] Ensembl hg38 (Hinxton) ENSG [Gene_View] ENSG [Sequence] chr [Contig_View] PRDM12 [Vega] ICGC DataPortal: ENSG TCGA cBioPortal: PRDM The embryonic vertebrate neural tube is divided along its dorsoventral (DV) axis into eleven molecularly discrete progenitor domains.
Each of these domains gives rise to distinct neuronal cell types; the ventral-most six domains contribute to motor circuits, while the five dorsal domains contribute to sensory circuits. Following the initial neurogenesis step, these domains also generate glial. PRDM Addgene Alerts Receive email alerts when new plasmids with this gene become available.
Log in to subscribe to Addgene Alerts. Description PR domain containing 12 Also known as PFM9 Species Homo sapiens Entrez ID MGC ID BC Plasmids containing this gene, or a homologous gene.
This gene encodes PRDM9 (PR/SET Domain 9), a zinc-finger DNA-binding protein with histone methyltransferase activity that is expressed in early meiotic prophase, during leptonema and .However, longitudinal characterization of the neurobehavioral deficits in many of these animal models is still lacking (Del Pino et al., ), which, given the extensive molecular and circuit.Prdm12 was also expressed in the spinal cord at E In addition, weak expression of Prdm12 was observed in the caudal forebrain and midbrain (Fig.
2B, blue and yellow arrowheads). At E, these two expression domains of Prdm12 in forebrain and midbrain became stronger (Fig. 2E, red, blue and yellow arrowheads, respectively).